|
| Status |
Public on Apr 25, 2014 |
| Title |
Wild type, 30μM arsenate, 1.5 hours. Rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Wild type, 30μM arsenate, 1.5 hours
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Col-0
genotype: Wild Type
supplement: Arsenate (30μM)
time: 1.5 hours
|
| Treatment protocol |
Plants were transferred to liquid medium supplemented with 30 μM As(V), 30 μM Pi, 1mM Pi (or not supplemented) for indicated times.
|
| Growth protocol |
Plants were grown on Johnson medium with 1 mM Pi (7 days), transferred to -Pi medium (2 days).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from three independent biological samples for each condition using the RNeasy Plant Mini Kit (Qiagen). Each RNA preparation was tested for degradation using the Agilent 2100 Bioanalyzer (Agilent technologies, Palo Alto, CA).
|
| Label |
Cy3
|
| Label protocol |
cDNA was Cy3-labeled with the Nimblegen One-Color DNA Labeling Kit (Roche).
|
| |
|
| Hybridization protocol |
Each microarray was hybridized with the Nimblegen Hybridization Kit, washed using the Nimblegen Wash Buffer Kit (both from Roche) and scanned at 2 μm double-pass at 16 bit of resolution.
|
| Scan protocol |
Scanned at 2 μm double-pass at 16 bit of resolution with the official Nimblegen scanner.
|
| Description |
Wild type plants supplemented with 30μM arsenate (1.5 hours). Replicate 1
|
| Data processing |
Data were analyzed through Robust Multi-array Analysis (RMA) with Nimblegen Scan software (Roche).
|
| |
|
| Submission date |
Jul 19, 2013 |
| Last update date |
Apr 25, 2014 |
| Contact name |
Juan Carlos Oliveros |
| Organization name |
CNB, CSIC
|
| Street address |
Darwin 3
|
| City |
Cantoblanco |
| State/province |
Madrid |
| ZIP/Postal code |
28049 |
| Country |
Spain |
| |
|
| Platform ID |
GPL13970 |
| Series (1) |
| GSE49037 |
WRKY6 Transcription Factor Restricts Arsenate Uptake and Transposon Activation in Arabidopsis |
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