|
| Status |
Public on Jul 01, 2007 |
| Title |
PCA17_localized prostate cancer |
| Sample type |
RNA |
| |
|
| Source name |
human prostate cancer of untreated patient
|
| Organism |
Homo sapiens |
| Characteristics |
70 percent tumor content
Age: 66
|
| Treatment protocol |
radical prostatectomy
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA with subsequent purification via RNeasy Columns
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the RiboAmp OA RNA 2 round amplification kit (Arcturus) starting from 1 ug RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from human prostate tissue with 90 percent tumor content
|
| Data processing |
The data were analyzed with the RMA algorithm in R using the quantile neromalization method
|
| |
|
| Submission date |
Jul 07, 2006 |
| Last update date |
Feb 23, 2007 |
| Contact name |
Oliver Christoph Stoss |
| E-mail(s) |
oliver.stoss@targos-gmbh.com
|
| Phone |
+49 561 980 4104
|
| Fax |
+49 561 980 6899
|
| URL |
http://www.targos-gmbh.de
|
| Organization name |
Targos GmbH
|
| Department |
Molecular Pathology
|
| Lab |
Bioinformatics
|
| Street address |
Moenchebergstr.41
|
| City |
Kassel |
| ZIP/Postal code |
34125 |
| Country |
Germany |
| |
|
| Platform ID |
GPL201 |
| Series (1) |
| GSE5377 |
Gene expression profiling of hormone resistant prostate cancer |
|
