|
| Status |
Public on Sep 25, 2019 |
| Title |
PD Patient # 14 |
| Sample type |
RNA |
| |
|
| Source name |
PD Patient # 14
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: Parkinson's disease
cell type: peripheral blood mononuclear cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Blood from control and PD subjects was collected in Vacutainer® CPT Cell Preparation Tubes (Becton Dickinson, Le Pont-de-Claix, France) and PBMC from human samples were isolated on a ficoll gradient. Total RNA from 5 x 106 cells was isolated using Rneasy mini-columns (Qiagen, Courtaboeuf, France). RNA concentration was measured by absorbance at 260nm using a Nanodrop ND-1000 spectrophotometer. RNA quality and integrity were checked using the RNA 6000 NanoChip kit on the Agilent 2100 Bioanalyzer (Agilent Technologies, Massy, France). All samples included in the study had a RIN>9.
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cya3) labeled cRNA was prepared from 1650 ng RNA using the One-Color Low RNA Input Linear Amplification PLUS kit (Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN), Valencia, CA). Dye incorporation and cRNA yield were with the NanoDrop ND-1000 Spectrophotometer.
|
| |
|
| Hybridization protocol |
Scanned on an Agilent G2505B scanner and Agilent-014850 whole Human genome microarrays 4x44K (Probe Name Version).
|
| Scan protocol |
Images were quantified using Agilent Feature Extraction Software (version A.9.5.3.1).
|
| Description |
Trasncriptomic profiling of peripheral blood mononuclear cells from patients with Parkinson's disease and control subjects
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 9.5.3.1 (Agilent) using default parameters (Grid: 014850_D_20060807) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
|
| |
|
| Submission date |
Jul 23, 2013 |
| Last update date |
Sep 25, 2019 |
| Contact name |
Marie-Christine Chartier-Harlin |
| Organization name |
INSERM
|
| Street address |
1 place de Verdun
|
| City |
Lille |
| ZIP/Postal code |
59045 |
| Country |
France |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE49126 |
Transcriptomic profiling of peripheral blood mononuclear cells from patients with Parkinson's disease and control subjects |
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