|
Status |
Public on Apr 29, 2014 |
Title |
ACC43 |
Sample type |
RNA |
|
|
Source name |
Frozen tissue of Adrenocortical carcinomas
|
Organism |
Homo sapiens |
Characteristics |
age (years): 52 gender: M cell type: Adrenocortical carcinoma
|
Growth protocol |
Samples stored at -80 C
|
Extracted molecule |
total RNA |
Extraction protocol |
Tumor samples (10 to 50 mg) were powdered under liquid nitrogen. RNA were extracted using RNAble (Eurobio, Courtaboeuf, France), followed by a clean-up step on RNAeasy columns (Qiagen, Courtaboeuf, France).
|
Label |
biotin
|
Label protocol |
The labeling of the extracted RNA was performed according to the manufacturer's instruction (Affymetrix)
|
|
|
Hybridization protocol |
Samples were hybridized with GeneChip Human Exon 2.0 ST Arrays (Affymetrix)
|
Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
Data processing |
Raw data were processed with the RMA normalisation (Bioconductor R affy package) + ComBat (R sva package ) to generate log2 normalized intensity signal. Data analysis and statistical evaluations were performed with customized R codes.
|
|
|
Submission date |
Jul 26, 2013 |
Last update date |
Apr 29, 2014 |
Contact name |
Nabila Elarouci |
E-mail(s) |
Nabila.Elarouci@ligue-cancer.net
|
Organization name |
Ligue Nationale contre le Cancer
|
Department |
Recherche
|
Lab |
Carte d'identité des tumeurs
|
Street address |
14 rue Corvisart
|
City |
Paris |
ZIP/Postal code |
75 013 |
Country |
France |
|
|
Platform ID |
GPL16686 |
Series (2) |
GSE49278 |
Expression profiling by array of 44 adrenocortical carcinomas |
GSE49280 |
Integrated genomic analyses of adrenocortical tumors (SNP array, DNA methylation, mRNA and miRNA expression). |
|