|
| Status |
Public on Mar 17, 2014 |
| Title |
H3-HC22 |
| Sample type |
RNA |
| |
|
| Source name |
neutrophils
|
| Organism |
Homo sapiens |
| Characteristics |
experiment: II
donors: H2
activators: Uninfected plasma
sample id: HC22
age (years): 55
gender: Female
bacterial identification: -
day of blood collection: 1
severity: -
cell type: neutrophil
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the QIAGEN MicroRNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with the Agilent Bioanalyser.
|
| Label |
Biotin
|
| Label protocol |
Standard Illumina labeling protocol.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Arrays were scanned using the Illumina HiScan and the standard Illumina scanning protocol.
|
| Data processing |
The data were preprocessed using R, which included the following steps: quantile normalization, flooring all intensities <10 to 10, log2 transformation, and PALO (present at least once) filter. PALO, which selects only transcripts with detection p-values < 0.01 in at least one sample, was performed to reduce background noise.
|
| |
|
| Submission date |
Aug 10, 2013 |
| Last update date |
Mar 17, 2014 |
| Contact name |
Damien Chaussabel |
| E-mail(s) |
DChaussabel@benaroyaresearch.org
|
| Organization name |
Baylor Institute for Immunology Research
|
| Street address |
3434 Live Oak
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75204 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE49756 |
A Transcriptomic Reporter Assay Employing Neutrophils to Measure Immunogenic Activity of Septic Patients' Plasma (Expt. 2) |
| GSE49758 |
A Transcriptomic Reporter Assay Employing Neutrophils to Measure Immunogenic Activity of Septic Patients' Plasma |
|
