|
Status |
Public on Sep 01, 2014 |
Title |
AC_chd, wnt3a and lrig3MO injected_rep2 |
Sample type |
RNA |
|
|
Source name |
animal caps injected with Chordin, Wnt3a mRNAs and Lrig3MO
|
Organism |
Xenopus laevis |
Characteristics |
injected with: Chordin, Wnt3a mRNAs and Lrig3MO tissue: animal caps
|
Treatment protocol |
Xenopus embryos were injected with mRNAs or MO in the animal pole at two-cell stage. The animal caps were dissected at stage 9 using forceps in 1×MMR on 1% agar plate.
|
Growth protocol |
Xenopus embryos were incubated in 0.2×MMR at 13°C and staged according to Nieuwkoop and Faber. The dissected animal caps were cultured in L-15 medium (67% L-15, 7.5 mM Tris-HCl pH7.5, 1 mg/ml BSA).
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using the TRIzol reagent according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated aRNA was synthesized through in vitro transcription according to the standard Affymetrix protocol (GeneChip® 3' IVT Express Kit user manual).
|
|
|
Hybridization protocol |
Following fragmentation, 10 μg of aRNA were hybridized for 16 hr at 45°C on GeneChip® Xenopus laevis 2.0 Genome Array (64 Format). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
Description |
Sample name: 8_chd_wnt3a_L3MO Gene expression data from animal caps injected with Chordin, Wnt3a mRNAs and Lrig3MO
|
Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
|
|
Submission date |
Aug 30, 2013 |
Last update date |
Sep 01, 2014 |
Contact name |
Hui Zhao |
E-mail(s) |
zhaohui@cuhk.edu.hk
|
Phone |
0085239431344
|
Organization name |
The Chinese University of Hong Kong
|
Department |
School of Biomedical Sciences
|
Street address |
The Chinese University of Hong Kong
|
City |
Shatin |
ZIP/Postal code |
000000 |
Country |
Hong Kong |
|
|
Platform ID |
GPL10756 |
Series (1) |
GSE50487 |
Identification of Lrig3 target genes in Xenopus animal caps injected with wnt3a and chordin |
|