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Sample GSM1231291 Query DataSets for GSM1231291
Status Public on Apr 11, 2014
Title SNB19 PARK2 siRNA#2 replicate 2
Sample type RNA
 
Source name SNB19 GBM cell line
Organism Homo sapiens
Characteristics cell line: SNB19
transfection: PARK2 siRNA#2
Treatment protocol siRNAs were transfected into cells in antibiotic-free medium using Lipofectamine RNAiMAX , medium was changed after 4–6 h and cells were harvested after 48 hours .
Growth protocol SNB19 cells were cultured in DMEM media supplemented with 10% FBS and penicillin-streptomycin at 37 °C in 5% CO2. SF539 cells were grown in MEM media with 10% FBX and penicillin-streptomycin at 37 °C in 5% CO2
Extracted molecule total RNA
Extraction protocol Total RNA was extracted by using Rneasy Plus Mini Kit (Qiagen). RNA quality was determined with the Nanodrop Spectrophotometer (Thermo).
Label Biotin
Label protocol Affymetrix protocol
 
Hybridization protocol Affymetrix protocol
Scan protocol Affymetrix protocol
Data processing data was normalized and analyzed using partek software and R packages. Specifically log2 transformation was used for data normalization.
 
Submission date Sep 13, 2013
Last update date Apr 12, 2014
Contact name Yongxing Gong
E-mail(s) gongy1@mskcc.org
Phone 6468882783
Organization name Momerial Sloan-Kettering Cancer Center
Street address 1275 York Avenue
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platform ID GPL571
Series (1)
GSE50864 PARK2 knockdown in 2 glioma cell lines

Data table header descriptions
ID_REF
VALUE quantile normalized

Data table
ID_REF VALUE
1007_s_at 10.9522
1053_at 9.1819
117_at 4.57778
121_at 7.53772
1255_g_at 2.69416
1294_at 5.77571
1316_at 6.1481
1320_at 4.73004
1405_i_at 2.91458
1431_at 3.42838
1438_at 5.46553
1487_at 7.30907
1494_f_at 4.62158
1598_g_at 8.69407
160020_at 6.71277
1729_at 8.76159
1773_at 5.34402
177_at 4.91847
179_at 7.72854
1861_at 7.08126

Total number of rows: 16376

Table truncated, full table size 301 Kbytes.




Supplementary file Size Download File type/resource
GSM1231291_SNB19_PARK2_siRNA_2_replicate_2.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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