|
| Status |
Public on Aug 31, 2014 |
| Title |
jmj30 jmj32, rep2 |
| Sample type |
RNA |
| |
|
| Source name |
jmj30 jmj32, 13 DAG, 29°C LD
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
genetic background: Col-0
tissue: seedling
genotype: jmj30-2 jmj32-1
phenotype: Flowered earlier than WT grown at similar condition
|
| Treatment protocol |
Plants were shift to elevated temperature conditions (29°C, LD) at 3 DAG. Samples were harvested at 13 DAG.
|
| Growth protocol |
The seeds of the indicated genotype were planted on soil, and grown at 22°C under long day (LD, 16 hour light, 8 hour dark) conditions until treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini kit (Qiagen Inc., Valencia, CA, USA) and DNA was removed by on-column DNaseI digestion with the RNase-Free DNase set (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed following NimbleGen standard operating protocol. See www.nimblegen.com.
|
| |
|
| Hybridization protocol |
Hybridization was performed following NimbleGen standard operating protocol. See www.nimblegen.com.
|
| Scan protocol |
Scanning was performed following NimbleGen standard operating protocol. See www.nimblegen.com.
|
| Description |
Total RNA from jmj30 jmj32 plants were used in this experiment. This is the second sample of the two biological replicates.
|
| Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
|
| |
|
| Submission date |
Oct 30, 2013 |
| Last update date |
Aug 31, 2014 |
| Contact name |
Toshiro Ito |
| E-mail(s) |
itot@tll.org.sg
|
| Organization name |
Temasek Life Sciences Laboratory
|
| Lab |
Plant Systems Biology
|
| Street address |
1 Research Link, National University of Singapore
|
| City |
Singapore |
| ZIP/Postal code |
117604 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL13970 |
| Series (2) |
| GSE51897 |
Gene expression analysis of jmj30 jmj32 mutant grown at 29°C under long day conditions |
| GSE51898 |
Gene expression analysis of overexpression and loss-of-function at JUMONJI30 |
|
