|
| Status |
Public on Apr 22, 2014 |
| Title |
Hs294T_AttRho_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Hs294T human melanoma cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Hs294T
treatment: AttRho
replicate: 2
|
| Treatment protocol |
Hs294T were serum starved for 48h and in the presence of 50 µmol/L Ilomastat or serum starved for 48 h and treated with the Rho activator Calpeptin 1 U/ml for the last 2 h of incubation. EphA2-overexpressing Hs294Tcells were serum starved for 48 h
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from Hs294T cells using RNeasy Plus Mini kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
cRNA were hybridized onto Human AffymetrixHuGene St 1.0 GeneChip array according to the standard Affymetrix protocol
|
| Scan protocol |
Scanning was performed according to the standard Affymetrix protocol
|
| Description |
Hs294T were serum starved for 48 h and treated with the Rho activator Calpeptin 1 U/ml for the last 2 h of incubation
|
| Data processing |
The data were analyzed with R/Bioconductor and RMA algorithm in affypackage was used as normalization method
|
| |
|
| Submission date |
Nov 09, 2013 |
| Last update date |
Apr 22, 2014 |
| Contact name |
Maurizio Callari |
| E-mail(s) |
maurizio.callari@cruk.cam.ac.uk
|
| Organization name |
Fondazione IRCCS Istituto Nazionale dei Tumori
|
| Street address |
Via Amadeo, 42
|
| City |
Milan |
| ZIP/Postal code |
20133 |
| Country |
Italy |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE52246 |
Mesenchymal to amoeboid transition is associated with stem-like features of melanoma cells |
|
