|
| Status |
Public on Nov 16, 2013 |
| Title |
Influenza challenge sample at 84 hours from subject H1N1_009 |
| Sample type |
RNA |
| |
|
| Source name |
RNA from peripheral human blood from adult participating in influenza challenge study
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: blood
challenge virus: H1N1
clinical phenotype: Symptomatic
total symptom score: 33
subject id: H1N1_009
time point: 84 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted from PaxGene Blood tubes as per the manufacturer's protocol.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated antisense strand cRNA was prepared according to the standard Affymetrix protocol from 2.5 ug total RNA utilizing peptide nucleic acids complimentary to globin mRNA to block amplification of those transcripts(GeneChip® 1 cycle labeling kit with Globin Reduction protocol (Affymetrix) Data was Generated at Expression Analysis, Inc (Durham, NC))
|
| |
|
| Hybridization protocol |
20 ug of antisense cRNA was hybridized for 16+ hr at 45C on GeneChip Human U133A array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using the Midi_euk2v3_450 protocol
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 3000 7G.
|
| Description |
Gene expression data at 84 hours from adult human volunteer H1N1_009 challenged with H1N1 virus who became symptomatic.
Healthy adult volunteers were inoculated intranasally with either influenza H3N2 or influenza H1N1 virus. Symptoms were recorded at standard intervals and a time course of peripheral blood was collected in RNA PaxGene tubes.
|
| Data processing |
ExpressionStat 5.0
|
| |
|
| Submission date |
Nov 15, 2013 |
| Last update date |
Nov 30, 2020 |
| Contact name |
Geoffrey S Ginsburg |
| E-mail(s) |
geoffrey.ginsburg@duke.edu
|
| Organization name |
Duke University Medical Center
|
| Department |
Genomic Medicine
|
| Lab |
Ginsburg
|
| Street address |
101 Science Dr, CIEMAS 2111, Box 3382
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27708 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE52428 |
Host gene expression signatures of influenza A H1N1 and H3N2 virus infection in adults |
|
| Relations |
| Reanalyzed by |
GSE162329 |
