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Sample GSM1273855 Query DataSets for GSM1273855
Status Public on Nov 24, 2014
Title Ly6CHi-SPL-SOD1/miR155-/-
Sample type RNA
 
Source name Mouse -B6/SJL-mSOD1G93A-miR-155-/-
Organism Mus musculus
Characteristics cell type: Splenic FACS sorted Ly6CHi monocytes
genotype/strain: B6SJL-Tg(SOD1-G93A)1Gur/J (miR-155-/-)
gender: female
Treatment protocol NA
Growth protocol NA
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using mirVanaTM miRNA isolation kit (Ambion) according to the manufacturer’s protocol
Label NA
Label protocol nCounter Mouse miRNA Assay Kit
 
Hybridization protocol according to the manufacturer’s protocol
Scan protocol according to the manufacturer’s protocol
Data processing Data was proccessed using nSolver, normalized to Positive controls, Housekeeping genes and negative controls
 
Submission date Nov 22, 2013
Last update date Nov 24, 2014
Contact name Oleg Butovsky
E-mail(s) obutovsky@rics.bwh.harvard.edu
Phone 1-617-525-5313
Organization name Brigham and Women's Hospital, Harvard Medical School
Department Center of Neurologic Diseases
Lab Dr. Oleg Butovsky
Street address 77 Avenue Louis Pasteur, Office 614
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL17873
Series (2)
GSE52668 miRNA profile in SOD-155 microglia
GSE52947 Targeting miR-155 restores abnormal microglia and attenuates disease in SOD1 mice

Data table header descriptions
ID_REF
VALUE Normalized mRNA transcripts copies per 100ng of total RNA

Data table
ID_REF VALUE
mmu-let-7g 341.29
mmu-let-7i 60.23
mmu-miR-1 7.53
mmu-miR-15b 416.58
mmu-miR-23b 38.9
mmu-miR-27b 38.9
mmu-miR-29b 143.04
mmu-miR-30a 57.72
mmu-miR-30b 80.3
mmu-miR-99a 52.7
mmu-miR-99b 30.11
mmu-miR-101a 22.59
mmu-miR-124 20.08
mmu-miR-125a-5p 37.64
mmu-miR-125b-5p 45.17
mmu-miR-126-5p 12.55
mmu-miR-126-3p 15.06
mmu-miR-127 25.1
mmu-miR-128 21.33
mmu-miR-130a 13.8

Total number of rows: 599

Table truncated, full table size 11 Kbytes.




Supplementary file Size Download File type/resource
GSM1273855_20120113_ad-sod_ad-sod_01.RCC.gz 5.2 Kb (ftp)(http) RCC
Processed data included within Sample table

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