|
| Status |
Public on Dec 14, 2013 |
| Title |
WT2_CD8anegDC |
| Sample type |
RNA |
| |
|
| Source name |
C57Bl/6
|
| Organism |
Mus musculus |
| Characteristics |
cell type: CD8a- DCs
genetic background: C57Bl/6
genotype: wt
tissue: Spleen
|
| Treatment protocol |
Mice were analyzed at steady-state unless indicated. For short-term ex vivo culture and stimulation, CD8a+ DCs were purified by cell sorting and plated in flat-bottom tissue-culture treated plates at a density of 105 per 200 microL; CD8a+ DCs were stimulated with 10 ng/ml GM-CSF (Peprotech) for 24 hours.
|
| Growth protocol |
Primary splenocytes were harvested by digestion with Collagenase B and DNAse1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Bone marrow pDCs were sorted as CD11c+SiglecH+B220+, splenic CD8a+ DCs were sorted as CD11c+MHCII+CD24+CD172a-B220-, splenic CD8a- DCs were sorted as CD11c+MHCII+CD172a+CD24-B220-
|
| Label |
biotin
|
| Label protocol |
Ambion WT Expression kit, Genechip WT Terminal Labeling and Controls Kit
|
| |
|
| Hybridization protocol |
Hybed onto Mouse Gene 1.0 ST Arrays (Affymetrix)
|
| Scan protocol |
standard Affymetrix protocol
|
| Data processing |
Data was analyzed using DNASTAR Arraystar software.
|
| |
|
| Submission date |
Dec 13, 2013 |
| Last update date |
Dec 17, 2013 |
| Contact name |
Kenneth M Murphy |
| E-mail(s) |
kmurphy@pathology.wustl.edu
|
| Organization name |
Washington University School of Medicine
|
| Department |
Pathology and Immunology
|
| Street address |
660 S. Euclid Ave.
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE53312 |
Microarray analysis of WT and L-Myc-deficient DC subsets |
|
