|
| Status |
Public on Sep 24, 2006 |
| Title |
Profiling of altered gene expression induced by 3 hrs zinc in human prostate cells |
| Sample type |
RNA |
| |
|
| Source name |
HPR-1 cell line
|
| Organism |
Homo sapiens |
| Characteristics |
Human prostatic cell line derived from normal prostate epithelial tissue (HPR-1)
|
| Biomaterial provider |
Dr. C.K. Choo, University of Hong Kong, China
|
| Treatment protocol |
Zinc effect was initiated with cells at 70-80% confluence in T-75 flasks (Corning, Corning, NY) which were synchronized by 24 hrs hormone-depletion before the introduction of zinc treatments. Hormone-free media were refreshed following starving process and zinc (1,500 ng /mL) was introduced for 3 hrs. Both media and Cells were collected concurrently. Pellets were immediately frozen and stored at -80 oC pending for RNA isolation.
|
| Growth protocol |
Cells were maintained and propagated in Keratinocyte-SFM supplemented with 1U/mL penicillin and streptomycin sulfate, EGF 0.005 mg/mL, and bovine pituitary extract 0.05 mg/mL in a 5% CO2 atmosphere at 37oC.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with the Qiagen (Valencia, CA) RNeasy Mini kit according to the manufacturer’s protocol.
|
| Label |
Chemiluminescent Fluorescence
|
| Label protocol |
Applied Biosystems Protocol
|
| |
|
| Hybridization protocol |
Applied Biosystems Protocol
|
| Scan protocol |
Applied Biosystems Protocol
|
| Description |
Zinc effect on gene expression of HPR-1 cells was evaluated using Applied Biosystems (Foster City, CA) human genomewise microarray chips V2.0. For each chip, 2µg total RNA from each sample was used for converting to cRNA. The synthesis of cRNA and a subsequent hybridization were completed by the Core Facility at UMBI (University of Maryland Biotechnology Institute, Baltimore, MD) according to the ABI standard protocol.
|
| Data processing |
Applied Biosystems 1700 Chemiluminescent Microarray Analyzer and 1700 Gene Expression Microarray System
|
| |
|
| Submission date |
Aug 21, 2006 |
| Last update date |
Aug 24, 2006 |
| Contact name |
Shufei Lin |
| E-mail(s) |
slin@umaryland.edu
|
| Phone |
410-706-7340
|
| Fax |
410-706-7340
|
| Organization name |
University of Maryland at Baltimore
|
| Department |
Biomedical Sciences
|
| Lab |
4D-08 Dental School
|
| Street address |
666 W. Baltimore Street
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21201 |
| Country |
USA |
| |
|
| Platform ID |
GPL2986 |
| Series (1) |
| GSE5590 |
Profiling of zinc altered gene expression in human prostate normal (HPR-1) and malignant (PC-3) cells |
|
