|
| Status |
Public on Sep 24, 2006 |
| Title |
Profiling of altered gene expression induced by 6 hrs of zinc in human prostate cells |
| Sample type |
RNA |
| |
|
| Source name |
HPR-1
|
| Organism |
Homo sapiens |
| Characteristics |
Human prostatic cell line derived from normal prostate epithelial tissue (HPR-1)
|
| Biomaterial provider |
Dr. C.K. Choo, University of Hong Kong, China
|
| Treatment protocol |
Zinc effect was initiated with cells at 70-80% confluence in T-75 flasks (Corning, Corning, NY) which were synchronized by 24 hrs hormone-depletion before the introduction of zinc treatments. Hormone-free media were refreshed following starving process and zinc (1,500 ng /mL) was introduced for 6 hrs. Both media and Cells were collected concurrently. Pellets were immediately frozen and stored at -80 oC pending for RNA isolation.
|
| Growth protocol |
Cells were maintained and propagated in Keratinocyte-SFM supplemented with 1U/mL penicillin and streptomycin sulfate, EGF 0.005 mg/mL, and bovine pituitary extract 0.05 mg/mL in a 5% CO2 atmosphere at 37oC.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with the Qiagen (Valencia, CA) RNeasy Mini kit according to the manufacturer?s protocol.
|
| Label |
Chemiluminescent Fluorescence
|
| Label protocol |
Applied Biosystems Protocol
|
| |
|
| Hybridization protocol |
Applied Biosystems Protocol
|
| Scan protocol |
Applied Biosystems Protocol
|
| Description |
Zinc effect on gene expression of HPR-1 cells was evaluated using Applied Biosystems (Foster City, CA) human genomewise microarray chips V2.0. For each chip, 2 microgram total RNA from each sample was used for converting to cRNA. The synthesis of cRNA and a subsequent hybridization were completed by the Core Facility at UMBI (University of Maryland Biotechnology Institute, Baltimore, MD) according to the ABI standard protocol.
|
| Data processing |
Applied Biosystems 1700 Chemiluminescent Microarray Analyzer and 1700 Gene Expression Microarray System
|
| |
|
| Submission date |
Aug 21, 2006 |
| Last update date |
Aug 24, 2006 |
| Contact name |
Shufei Lin |
| E-mail(s) |
slin@umaryland.edu
|
| Phone |
410-706-7340
|
| Fax |
410-706-7340
|
| Organization name |
University of Maryland at Baltimore
|
| Department |
Biomedical Sciences
|
| Lab |
4D-08 Dental School
|
| Street address |
666 W. Baltimore Street
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21201 |
| Country |
USA |
| |
|
| Platform ID |
GPL2986 |
| Series (1) |
| GSE5590 |
Profiling of zinc altered gene expression in human prostate normal (HPR-1) and malignant (PC-3) cells |
|
