|
Status |
Public on May 15, 2014 |
Title |
KM-final |
Sample type |
RNA |
|
|
Source name |
PBMCs from CIS patient after IFNb-1a in-vivo treatment
|
Organism |
Homo sapiens |
Characteristics |
tissue: PBMCs treatment: after IFNb-1a in-vivo treatment
|
Treatment protocol |
6x107 PBMCs were separated from 6 CIS patients at baseline and after 12 months of IFNb-1a (44 ug s.q. tiw) treatment.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
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|
|
Hybridization protocol |
15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
|
Scan protocol |
The arrays were scanned with the Hewlett Packard GeneArray Scanner.
|
Data processing |
Affymetrix GeneChip® Microarray Suite 5.0 software
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|
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Submission date |
Dec 30, 2013 |
Last update date |
Jul 21, 2014 |
Contact name |
Silva Markovic-Plese |
E-mail(s) |
markovics@neurology.unc.edu
|
Phone |
919 966 3701
|
Organization name |
University of North Carolina at Chapel Hill
|
Street address |
105 Mason Farm Rd
|
City |
Chapel Hill |
State/province |
NC |
ZIP/Postal code |
27516 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE53716 |
IFNb-1a in-vivo treatment induces the expression and signaling of IFNAR1 and inhibits Th17 responses in PBMCs derived from CIS patients |
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