|
| Status |
Public on Jul 14, 2014 |
| Title |
Superficial temporal artery, sample 3 |
| Sample type |
RNA |
| |
|
| Source name |
Superficial temporal artery, sample 3
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Superficial temporal artery
status: Superficial temporal artery
Sex: Female
age: 34
|
| Treatment protocol |
All the specimens were snap-frozen in liquid nitrogen, and stored at -80 °C.
|
| Growth protocol |
In case of ruptured intracranial aneurysms, all resections were done within 24 hours after initial subarachnoid hemorrhage.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with the TRIzol reagent (Invitrogen, Carlsbad, CA, USA).
|
| Label |
Cy3
|
| Label protocol |
500 ng of total RNA was amplified and labeled with the Agilent Quick Amp Labeling Kit according to the manufacturers instructions.
|
| |
|
| Hybridization protocol |
1.65 ug of cyanine 3-CTP (Cy3)-labeled cRNA sample was used for 17-hour hybridization at 65 C.
|
| Scan protocol |
The Agilent DNA microarray scanner (model G2505A) was used. Signal intensity was quantified from the scanned image by using Feature Extraction Software version 9.1 (Agilent technologies)
|
| Description |
Gene expression in superficial temporal artery
|
| Data processing |
The signal intensities were corrected for the background intensities with the normexp method, followed by the quantile normalization across arrays with the limma software.
|
| |
|
| Submission date |
Jan 14, 2014 |
| Last update date |
Jul 14, 2014 |
| Contact name |
Hirofumi Nakaoka |
| E-mail(s) |
hnakaoka@nig.ac.jp
|
| Phone |
81-055-981-6795
|
| Organization name |
National Institute of Genetics
|
| Department |
Department of Human Genetics
|
| Street address |
Yata 1111
|
| City |
Mishima |
| State/province |
Shizuoka |
| ZIP/Postal code |
411-8540 |
| Country |
Japan |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE54083 |
Gene expression profiling of intracranial aneurysms |
|
