|
| Status |
Public on Feb 20, 2008 |
| Title |
NBTII-FGFR1 transfected cells, FGF1 stimulation 2h rep1 |
| Sample type |
RNA |
| |
|
| Source name |
NBTII-FGFR1 cells
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain : NBTII-FGFR1 ; stimulation : FGF1 ; time : 2 hours
|
| Treatment protocol |
FGF1 stimulation (20ng/ml) + 50µl/ml heparine
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA PLUS™ extraction Kit (Bioprobes Systems)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Affymetrix protocol
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from NBTII-FGFR1 cells activated by FGF-1 ligand
|
| Data processing |
The data were analyzed with RMA global normalization procedure
|
| |
|
| Submission date |
Aug 29, 2006 |
| Last update date |
Aug 20, 2007 |
| Contact name |
Marianne Tuefferd |
| E-mail(s) |
tuefferd@vjf.inserm.fr
|
| Organization name |
INSERM
|
| Street address |
16 avenue Paul Vaillant Couturier
|
| City |
Villejuif cedex |
| ZIP/Postal code |
94807 |
| Country |
France |
| |
|
| Platform ID |
GPL341 |
| Series (1) |
| GSE5664 |
gene profiling in carcinoma cell during EMT |
|
