|
| Status |
Public on Dec 31, 2014 |
| Title |
3_KO_2 |
| Sample type |
RNA |
| |
|
| Source name |
Parp-2 KO_bone marrow cells
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
genotype/variation: Parp-2-/-
cell type: sorted Ter119+CD71+ bone marrow cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted following Quiagen protocol
|
| Label |
biotin
|
| Label protocol |
100ng of total RNA from each sample was processed, labelled according to the Affymetrix GeneChip® Whole Transcript Sense Target Labelling Assay
|
| |
|
| Hybridization protocol |
Sample was hybridized to Affymetrix Mouse Gene 1.0 ST array using a Affymetrix GeneChip Hybridization Oven 645
|
| Scan protocol |
GeneChip was scanned using Affymetrix GeneChip Scanner 3000 7G
|
| Data processing |
Data were analyzed using the RMA algorithm and then LIMMA was applied to calculate significant differential expression between samples. This was performed in R (v 3.0.2) with aroma.affymetrix and bioconductor packages.
|
| |
|
| Submission date |
Feb 11, 2014 |
| Last update date |
Oct 19, 2023 |
| Contact name |
Júlia Perera-Bel |
| E-mail(s) |
mardata-bu@researchmar.net
|
| Organization name |
Hospital del Mar Research Institute
|
| Department |
MARData-BU
|
| Street address |
Doctor Aiguader, 88
|
| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE54864 |
Parp-2 is required for efficient erythropoiesis in mice by limiting replicative stress in erythroid progenitors |
|
