|
| Status |
Public on Feb 13, 2014 |
| Title |
M464 Wt |
| Sample type |
RNA |
| |
|
| Source name |
Wt
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6
gender: male
age: 4-month-old
genotype/variation: Wt
tissue: Skeletal muscle (quadriceps)
|
| Treatment protocol |
Quadriceps muscles were extracted and directly frozen in liquid nitrogen
|
| Growth protocol |
Mice were kept under a 12h dark-light period and provided with a standard chow-diet and water ad libitum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from quadriceps muscles was extracted using a protocol combining TRIzol reagent (Invitrogen) and RNAeasy® minikit columns (Qiagen) following manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
25 ng total RNA per sample was processed using isothermal amplification SPIA Biotin System (NuGEN Technologies, Inc.)
|
| |
|
| Hybridization protocol |
2.2 µg of cDNA was hybridized per microarray for 16 hours of hybridization at 45ºC, washing and staining of microarrays was performed using a Fluidics Station 450 (Affymetrix, Santa Clara, CA)
|
| Scan protocol |
GeneChips were scanned in a GeneChip Scanner 3000 (Affymetrix, Santa Clara, CA)
|
| Description |
M464 - QV RNA
Gene expression data from quadriceps muscle of 4-month-old male mouse
|
| Data processing |
Arrays were processed in Bioconductor, using RMA background correction, quantile normalization and RMA summarization.
|
| |
|
| Submission date |
Feb 12, 2014 |
| Last update date |
Feb 13, 2014 |
| Contact name |
Oscar Reina Garcia |
| E-mail(s) |
oscar.reina@irbbarcelona.org
|
| Organization name |
IRB Barcelona
|
| Department |
Biostatistics and Bioinformatics
|
| Street address |
C/Baldiri Reixac 10
|
| City |
Barcelona |
| State/province |
Barcelona |
| ZIP/Postal code |
08028 |
| Country |
Spain |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE54917 |
Mus musculus gene expression profiling for Wt, SKM-Tg, C and SKM-KO in Affymetrix 430PM strip arrays |
|
