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Status |
Public on Feb 13, 2014 |
Title |
M464 Wt |
Sample type |
RNA |
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Source name |
Wt
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Organism |
Mus musculus |
Characteristics |
strain: C57Bl/6 gender: male age: 4-month-old genotype/variation: Wt tissue: Skeletal muscle (quadriceps)
|
Treatment protocol |
Quadriceps muscles were extracted and directly frozen in liquid nitrogen
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Growth protocol |
Mice were kept under a 12h dark-light period and provided with a standard chow-diet and water ad libitum
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Extracted molecule |
total RNA |
Extraction protocol |
RNA from quadriceps muscles was extracted using a protocol combining TRIzol reagent (Invitrogen) and RNAeasy® minikit columns (Qiagen) following manufacturer's instructions
|
Label |
biotin
|
Label protocol |
25 ng total RNA per sample was processed using isothermal amplification SPIA Biotin System (NuGEN Technologies, Inc.)
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Hybridization protocol |
2.2 µg of cDNA was hybridized per microarray for 16 hours of hybridization at 45ºC, washing and staining of microarrays was performed using a Fluidics Station 450 (Affymetrix, Santa Clara, CA)
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Scan protocol |
GeneChips were scanned in a GeneChip Scanner 3000 (Affymetrix, Santa Clara, CA)
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Description |
M464 - QV RNA Gene expression data from quadriceps muscle of 4-month-old male mouse
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Data processing |
Arrays were processed in Bioconductor, using RMA background correction, quantile normalization and RMA summarization.
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|
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Submission date |
Feb 12, 2014 |
Last update date |
Feb 13, 2014 |
Contact name |
Oscar Reina Garcia |
E-mail(s) |
oscar.reina@irbbarcelona.org
|
Organization name |
IRB Barcelona
|
Department |
Biostatistics and Bioinformatics
|
Street address |
C/Baldiri Reixac 10
|
City |
Barcelona |
State/province |
Barcelona |
ZIP/Postal code |
08028 |
Country |
Spain |
|
|
Platform ID |
GPL11180 |
Series (1) |
GSE54917 |
Mus musculus gene expression profiling for Wt, SKM-Tg, C and SKM-KO in Affymetrix 430PM strip arrays |
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