|
| Status |
Public on May 16, 2014 |
| Title |
PC3 cells stimulated with LPA, Rep2 |
| Sample type |
RNA |
| |
|
| Source name |
PC3 cells stimulated with LPA
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: PC3 cells (obtained from ATCC)
agent: LPA
|
| Treatment protocol |
The cells were serum starved for 24h and then stimulated with LPA (1µM) for 45min.
|
| Growth protocol |
The MDA-MB-231, MCF7, PC3 cells were cultured in DMEM, EMEM, F-12K Nutrient Medium respectively, suplemented with 10% FBS and 1% Penicillin/streptomycin at 37°C in a 5% CO2 incubator
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy kit (QIAGEN) as per manufacturer's protocol
|
| Label |
Biotin
|
| Label protocol |
Total RNA (250ng) was amplified and biotin-labeled using GeneChip® 3' IVT Express target labeling kit as per the protocol from Affymetrix
|
| |
|
| Hybridization protocol |
Hybridization was performed following Affymetrix protocol. 10µg of labeled cRNA was fragmented and denatured in hybridization buffer, then hybridized on chip during 16h at 45°C with contant mixing by rotation at 60 rpm in an Genechip hybridization oven 640 (Affymetrix)
|
| Scan protocol |
The arrays were scanned using the confocal laser (Genechip scanner 3000, Affymetrix)
|
| Description |
Transcriptomic data from PC3 cells stimulated with LPA
PC3+LPA_2
|
| Data processing |
The CEL files were generated using the Affymetrix GeneChip Command Console (AGCC) software 3.0. The obtained data were normalized with affymetrix Expression Console software using MAS5 statistical algorithm
|
| |
|
| Submission date |
Mar 27, 2014 |
| Last update date |
May 16, 2014 |
| Contact name |
Olivier Peyruchaud |
| E-mail(s) |
olivier.peyruchaud@inserm.fr
|
| Phone |
0033478785738
|
| Organization name |
INSERM U1033
|
| Lab |
Pathophysiology, diagnosis and treatment of bone disease
|
| Street address |
UFR de medicine Lyon Est (Domaine Laennec), 7-11 Rue Guillaume Paradin
|
| City |
Lyon |
| State/province |
Rhone Alpes |
| ZIP/Postal code |
69372 |
| Country |
France |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE56265 |
Transcriptomic analysis of human breast and prostate cancer cell lines on lysophosphatidic acid (LPA) stimulation |
|
