|
| Status |
Public on Jun 01, 2014 |
| Title |
P3HR1_miR28_B1_12h |
| Sample type |
RNA |
| |
|
| Source name |
Burkitt lymphoma cell line stably expressing the doxycycline-inducible pRTS1-GFP vector carrying the miR-28 precursor (bulk population B) and induced by addition of 1ug/ml doxycycline for 12h.
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: P3HR1 Burkitt lymphoma cell line
bulk population: B
treatment: Doxycycline [0.1ug/ml], 12h
genotype/variation: miR28
time: 12h
|
| Growth protocol |
cells were grown in IMDM medium supplemented with 10% FCS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacture's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on the HG-U133 Plus2.0 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Mar 27, 2014 |
| Last update date |
Jun 01, 2014 |
| Contact name |
Riccardo Dalla-Favera |
| E-mail(s) |
rd10@cumc.columbia.edu
|
| Organization name |
Columbia University
|
| Street address |
1130 St Nicholas Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE56268 |
miR-28 expression in the Burkitt lymphoma cell line P3HR1 |
|
