|
| Status |
Public on Mar 29, 2014 |
| Title |
double_mutant_leukemia_lymph_node_2 |
| Sample type |
RNA |
| |
|
| Source name |
p53 -/-; Prkdc scid/scid double-mutant pre-B lymph node cells
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: mixed 129/Sv and BALB/C
genotype/variation: p53 -/-; Prkdc scid/scid double-mutant
cell type: B-cell leukemia cells, arrested in pre-B-like stage
|
| Growth protocol |
Cells were isolated ex vivo from murine lymph nodes or cultured in vitro.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were isolated from murine lymph nodes with mechanical and protease disruption to generate a single cell suspension for flow sorting. After sorting, cells were lysed in TRIzol and RNA was extracted. Leukemic cell samples were a minimum of 85% CD19 positive by flow cytometry.
|
| Label |
biotin
|
| Label protocol |
cRNA was generated and labelled with biotin using the standard Affymetrix protocols, and hybridized to the Affymetrix Mouse Genome 430 2.0 array.
|
| |
|
| Hybridization protocol |
Hyb/wash was EukGE-WS2V4 protocol, using Affymetrix automated fluidics station, TCAG, Sick Kids.
|
| Scan protocol |
Arrays scanned on Affymetrix GeneChip Scanner 3000 by TCAG, Sick Kids.
|
| Description |
Leukemic blasts from double-knockout p53/PRKDC mice.
|
| Data processing |
Scaling target signal was 150, used GCOS and MAS5.0 scaling for normalization.
|
| |
|
| Submission date |
Mar 28, 2014 |
| Last update date |
Mar 31, 2014 |
| Contact name |
Paul Kowalski |
| Organization name |
The Hospital for Sick Children
|
| Department |
Developmental and Stem Cell Biology
|
| Lab |
Cynthia Guidos
|
| Street address |
PGCRL - 686 Bay St
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 0A4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE56345 |
Therapeutic potential of spleen tyrosine kinase inhibition for treatment of high-risk precursor B-cell acute lymphoblastic leukemia |
|
