Data table header descriptions |
ID_REF |
|
VALUE |
same as UNF_VALUE but with flagged values removed |
Dia. |
Diameter of the spot |
F1 Median |
Intensity target sample |
F1 Mean |
Mean signal intensity target sample |
F1 SD |
Standard deviation of the intensity |
B1 Median |
Median background of target sample |
B1 Mean |
Mean background of target sample |
B1 SD |
Standard deviation of the background |
% > B1+1SD |
Percentage of pixels that have a higher intensity than the background plus 1 SD |
% > B1+2SD |
Percentage of pixels that is higher than the backgound plus 2 SD |
F1 % Sat. |
Percentage saturated pixels |
F2 Median |
Median Signal intensity reference sample |
F2 Mean |
Intensity reference sample |
F2 SD |
Standard deviation of the intensity |
B2 Median |
Background of reference sample |
B2 Mean |
Background of reference sample |
B2 SD |
Deviation of the background |
% > B2+1SD |
Background plus 1 SD |
% > B2+2SD |
Percentage of pixels that is higher than the backgound plus 2 SD |
F2 % Sat. |
Percentage saturated pixels |
Ratio of Medians |
Ratio of median intensities |
Ratio of Means |
Ratio of mean intensities |
Median of Ratios |
Mediam of ratios |
Mean of Ratios |
Mean of ratios |
Ratios SD |
Standard deviation |
Rgn Ratio |
Regression ratio is determined by computing a linear regression between the population of pixels represented by wavelength 1 and wavelength 2 |
Rgn Rē |
Coefficient of determination provides a measure of the level of accuracy of the fit of the linear regression curve |
F Pixels |
Number of pixels in the foreground |
B Pixels |
Number of pixels in the background |
Sum of Medians |
Sum of medians |
Sum of Means |
Sum of means |
Log Ratio |
Log ratio |
F1 Median - B1 |
Median intensity of target minus the local background |
F2 Median - B2 |
Median intensity of the reference sample minus the local background |
F1 Mean - B1 |
Mean intensity of target minus the local background |
F2 Mean - B2 |
Mean intensity of the reference sample minus the local background |
Flags |
Negative number means the spot is bad or not found |
UNF_VALUE |
Background corrected intensity of the target sample normalized by VSN |