|
| Status |
Public on Dec 02, 2014 |
| Title |
day 30, infected_3 |
| Sample type |
RNA |
| |
|
| Source name |
day 30, infected
|
| Organism |
Mus musculus |
| Characteristics |
infection: Mycobacterium tuberculosis
strain: C57BL/6
tissue: lung
|
| Treatment protocol |
Mycobacterium tuberculosis infected mouse were treated with 500 mg/Kg of metformin starting day 7 post-infection for 30 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was performed according to the miRVana miRNA isolation kit. Total RNA integrity was assessed by Agilent Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the Epicentre TargetAmp Nano-g Biotin-aRNA Labelling kit for Illumina system.
|
| |
|
| Hybridization protocol |
750ng of labelled cRNAs were hybridized to the Illumina mouse arrays for 16 hours at 58°C and the arrays were washed and stained according to the Illumina Wash Protocol
|
| Scan protocol |
The mouse chips were scanned using BeadArray Scanner 500GX and the images were analyzed using GenomeStudio Gene Expression v 1.9.0
|
| Description |
I_d30_3
|
| Data processing |
The data were normalised using quantile normalisation with R package lumi.
|
| |
|
| Submission date |
May 05, 2014 |
| Last update date |
Dec 02, 2014 |
| Contact name |
jinmiao chen |
| E-mail(s) |
chen_jinmiao@immunol.a-star.edu.sg
|
| Organization name |
Singapore Immunology Network
|
| Street address |
8A Biomedical Grove, #04-06 Immunos
|
| City |
Singapore |
| ZIP/Postal code |
138648 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE57275 |
Genome-wide analysis of gene expression in lung cells from metformin treated Mycobacterium tuberculosis infected mice |
|
