|
| Status |
Public on Apr 01, 2007 |
| Title |
UCB Day 2 sample |
| Sample type |
other |
| |
|
| Source name |
Fresh UCB CD34 cells
|
| Organism |
Homo sapiens |
| Characteristics |
Donated cord blood from informed consent subjects
|
| Biomaterial provider |
Cell Sciences (Cygenics)
|
| Treatment protocol |
Cord blood mononuclear cells were extracted by standard ficoll method and CD34 cells were isolated from mononuclear cells by flow cytometry using mouse anti-human CD34 antibody (BD Biosciences)
|
| Growth protocol |
The fresh CD34 cells were subjected to ex vivo erythroid differentiation culture as described in:
Carlile, G.W., Smith, D.H., and Wiedmann, M. 2004. Caspase-3 has a nonapoptotic function in erythroid maturation. Blood 103: 4310-4316.
|
| Extracted molecule |
other |
| Extraction protocol |
miRNA extracted from CD34 cells using Ambion miRNA Isolation Kit (Cat#1560)
|
| Label |
Cy3 (Amersham Pharmacia)
|
| Label protocol |
Labeling protocol as described in Ambion miRNA Labeling Kit (Cat#1562)
|
| |
|
| Hybridization protocol |
Hybridization protocols as described in Ambion Probeset (Cat#1561v1) and Ambion miRNA Labeling Kit (Cat#1562)
|
| Scan protocol |
Processed slides were scanned using the microarray scanner GenePix 4000B (Axon), with the lasers power set at maximum with fixed PMT value and a scan resolution of 10 um.
|
| Description |
miRNA expression profiling was performed on ex vivo differentiating erythroid cultures derived from human UCB-CD34 cells. Day 0 samples were freshly isolated CD34 cells. This is used as the baseline for comparison with ex vivo induced erythroid differentiation cultures from Day 2, 4, 6, 9 and 12.
|
| Data processing |
Data in VALUE column are median signal intensity after background subtraction, and normalized by dividing with the average signal (after background subtraction) of the sample
|
| |
|
| Submission date |
Oct 09, 2006 |
| Last update date |
Mar 30, 2007 |
| Contact name |
Meng Ling Choong |
| Organization name |
Experimental Therapeutics Centre
|
| Department |
Drug Discovery
|
| Lab |
Assay Development Group
|
| Street address |
31 Biopolis Way, #03-01 Nanos
|
| City |
Singapore |
| ZIP/Postal code |
138669 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL4400 |
| Series (1) |
| GSE6000 |
miRNA expression during UCB-CD34 cells erythropoiesis |
|
