|
| Status |
Public on Jun 04, 2014 |
| Title |
SmoWT-MB_DMSO_1 |
| Sample type |
RNA |
| |
|
| Source name |
SmoWT-MB_DMSO
|
| Organism |
Mus musculus |
| Characteristics |
cell line: SmoWT-MB
cell type: Ptch-deficient mouse medulloblastoma cell line
treated with: DMSO for 6hrs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Sample 1.AVG_Signal
|
| Data processing |
Rank-invariant normalized data was generated using GenomeStudio v1.9.0 and converted to .gct file format, which was then collapsed to gene symbols using the GSEA desktop application (http://www.broadinstitute.org/gsea/index.jsp).
|
| |
|
| Submission date |
Jun 03, 2014 |
| Last update date |
Jun 04, 2014 |
| Contact name |
Yujie Tang |
| Organization name |
Stanford University School of Medicine
|
| Department |
Neurology
|
| Street address |
265 Campus Dr SIM1 G3055
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL18752 |
| Series (1) |
| GSE58185 |
Targeting the epigenetic regulation of Hedgehog transcriptional output through BET bromodomain inhibition |
|
