|
| Status |
Public on Aug 10, 2014 |
| Title |
RA5_SFbl_4h |
| Sample type |
RNA |
| |
|
| Source name |
Fibroblasts of RA patient
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: IL1
patient: 5
time point: 4
|
| Treatment protocol |
At the end of the fourth passage, the SFB were washed in serum-free Dulbeccos modified Eagle’s medium (DMEM) and then stimulated with 10 ng/ml of either IL-1β or PDGF-D
|
| Growth protocol |
Negative purification of primary SFBs from RA patients (purity: > 98%) was performed as previously described by Kinne et al
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
At each time point 4 the medium was removed and the cells were harvested after treatment with trypsin (0.25% in Versene; Invitrogen, Karlsruhe, Germany). After washing with phosphate-buffered saline, cells were lysed with RLT buffer (Qiagen, Hilden, Germany) and frozen at -70◦C.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
SFB from RA treated with IL1
|
| Data processing |
The data were RMA normalized.
|
| |
|
| Submission date |
Jun 03, 2014 |
| Last update date |
Aug 10, 2014 |
| Contact name |
Peter Kupfer |
| E-mail(s) |
peter.kupfer@hki-jena.de
|
| Organization name |
HKI Jena
|
| Street address |
Beutenbergstrasse 11a
|
| City |
Jena |
| ZIP/Postal code |
07745 |
| Country |
Germany |
| |
|
| Platform ID |
GPL15445 |
| Series (1) |
| GSE58203 |
Stimulation of RA SFBs with IL1 or PDGF-D |
|
