|
| Status |
Public on Jul 12, 2014 |
| Title |
Testis_TgMsi2_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse testis
|
| Organism |
Mus musculus |
| Characteristics |
strain: B6xCBA
age: 3 months
tissue: testis
genotype/variation: TgMsi2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from samples using a QIAGEN RNeasy Mini Kit (Hilden, Germany). The quality and quantity of mouse total RNA was ascertained on the Agilent Bioanalyser 2100.
|
| Label |
Cy3
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
comparison 2
|
| Data processing |
GenomeStudio was used to convert the signal on the array into a TXT file for analysis
|
| |
|
| Submission date |
Jul 11, 2014 |
| Last update date |
Jul 12, 2014 |
| Contact name |
Jessie M Sutherland |
| E-mail(s) |
jessie.sutherland@newcastle.edu.au
|
| Organization name |
University of Newcastle
|
| Street address |
University Drive
|
| City |
Callaghan |
| ZIP/Postal code |
2304 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE59358 |
Microarray analysis of differential gene expression of whole testis extracts from transgenic Musashi-2 and transgenic Musashi-1 over-expression mice |
|
