|
| Status |
Public on Jul 12, 2014 |
| Title |
Gfi-1 -/-; Id2 +/- mouse BMC 4 |
| Sample type |
RNA |
| |
|
| Source name |
Gfi-1 knockout mouse bone marrow cells with Id2 reduction
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57/Bl6
tissue: bone marrow
genotype: Gfi-1 -/-; Id2 +/-
|
| Treatment protocol |
No treatment
|
| Growth protocol |
All mice were backcrossed onto C57/Bl6 backgounds. Animals were treated in accordance with the Guidelines of the Frederick National Laboratory for Cancer Research Animal Care and Use Committee (ACUC) in accordance with the Guide for Care and Use of Laboratory Animals.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified from whole bone marrow cells using RNeasy kits (QIAGEN Inc., Valencia, CA) according to the manufacturer's instructions.
|
| Label |
none
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2007, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized for 16 hr at 45C on GeneChip Mouse Gene 1.0 ST Array.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from total mouse bone marrow cells
|
| Data processing |
The data were analyzed with Partek Genomics Suite version 6.6 using Affymetrix default analysis settings and global scaling as normalization method. The genes listed were selected based on the criteria that their expression changed by ≥ 1.5 fold that was statistically significant as indicated by a p-value < 0.05.
RT-PCR data for 12 selected genes from the same genotypes are available on the series level.
|
| |
|
| Submission date |
Jul 11, 2014 |
| Last update date |
Jul 12, 2014 |
| Contact name |
Jonathan R Keller |
| E-mail(s) |
kellerjo@mail.nih.gov
|
| Phone |
301-846-1461
|
| Organization name |
Center for Cancer Research, National Cancer Institute, NIH
|
| Department |
Mouse Cancer Genetics Program
|
| Lab |
Hematopoiesis and Stem Cell Biology Section
|
| Street address |
1050 Boyles St. P.O. Box B (Building 560, Room 12-03)
|
| City |
Frederick |
| State/province |
MD |
| ZIP/Postal code |
21702 |
| Country |
USA |
| |
|
| Platform ID |
GPL10740 |
| Series (1) |
| GSE59360 |
Whole gene expression data from Gfi-1-/- and Gfi-1-/-;Id2+/- mouse bone marrow cells |
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