|
Status |
Public on Oct 06, 2015 |
Title |
human serum donor 4 |
Sample type |
RNA |
|
|
Source name |
PBMC
|
Organism |
Homo sapiens |
Characteristics |
sample type: human serum donor
|
Treatment protocol |
PBMCs were treated according to protocol of Vissers et al.
|
Growth protocol |
PBMCs isolated according to protocol of Vissers et al. Journal of Visualized Experiments 2013.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using the RNeasy Mini kit (Qiagen) and DNA was removed with Turbo DNase (DNA-free kit, Ambion).
|
Label |
Cy3
|
Label protocol |
Labeling was performed following Nimblegen's standard operating protocol. See www.nimblegen.com.
|
|
|
Hybridization protocol |
Hybridization was performed following Nimblegen's standard operating protocol. See www.nimblegen.com.
|
Scan protocol |
Scanning was performed following Nimblegen's standard operating protocol. See www.nimblegen.com.
|
Data processing |
The raw data (.pair file) was subjected to RMA quantile normalization in Arraystar.
|
|
|
Submission date |
Jul 14, 2014 |
Last update date |
Oct 06, 2015 |
Contact name |
Marloes Vissers |
E-mail(s) |
marloes.Vissers@radboudumc.nl
|
Organization name |
Radboud university medical center
|
Department |
Department of Pediatrics
|
Lab |
Laboratory of Pediatric Infectious Diseases
|
Street address |
Geert Grooteplein Zuid 10
|
City |
Nijmegen |
ZIP/Postal code |
6525 GA |
Country |
Netherlands |
|
|
Platform ID |
GPL18943 |
Series (1) |
GSE59391 |
Deciphering the gene expression response of human immune cells to stimulation with RSV in the context of antibodies |
|