|
| Status |
Public on Oct 06, 2015 |
| Title |
RSV donor 4 |
| Sample type |
RNA |
| |
|
| Source name |
PBMC
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: RSV donor
|
| Treatment protocol |
PBMCs were treated according to protocol of Vissers et al.
|
| Growth protocol |
PBMCs isolated according to protocol of Vissers et al. Journal of Visualized Experiments 2013.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini kit (Qiagen) and DNA was removed with Turbo DNase (DNA-free kit, Ambion).
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed following Nimblegen's standard operating protocol. See www.nimblegen.com.
|
| |
|
| Hybridization protocol |
Hybridization was performed following Nimblegen's standard operating protocol. See www.nimblegen.com.
|
| Scan protocol |
Scanning was performed following Nimblegen's standard operating protocol. See www.nimblegen.com.
|
| Data processing |
The raw data (.pair file) was subjected to RMA quantile normalization in Arraystar.
|
| |
|
| Submission date |
Jul 14, 2014 |
| Last update date |
Oct 06, 2015 |
| Contact name |
Marloes Vissers |
| E-mail(s) |
marloes.Vissers@radboudumc.nl
|
| Organization name |
Radboud university medical center
|
| Department |
Department of Pediatrics
|
| Lab |
Laboratory of Pediatric Infectious Diseases
|
| Street address |
Geert Grooteplein Zuid 10
|
| City |
Nijmegen |
| ZIP/Postal code |
6525 GA |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL18943 |
| Series (1) |
| GSE59391 |
Deciphering the gene expression response of human immune cells to stimulation with RSV in the context of antibodies |
|
