|
| Status |
Public on Jan 24, 2007 |
| Title |
Samp8 Hippocampus Old Sample 3 |
| Sample type |
RNA |
| |
|
| Source name |
SAMP8 Hippocampus Old Sample 3
|
| Organism |
Mus musculus |
| Characteristics |
SAMP8/Ta senescence-accelerated mouse line
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Hybridizations were performed on Affymetrix MG_U74Av2 GeneChips for 16 hr at 50C at a final cRNA concentration of 0.66 ug/ul. GeneChips were washed and stained in the Salk Megawasher.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix/Agilent GeneArray Scanner.
|
| Description |
Gene expression data from hippocampus of one old SAMP8/Ta mouse
|
| Data processing |
Data were analyzed using the Teragenomics Relational Database http://www.teragenomics.com/public/prodNServices_absolutePrecis.asp
|
| |
|
| Submission date |
Nov 07, 2006 |
| Last update date |
Jan 24, 2007 |
| Contact name |
Jennifer Greenhall |
| E-mail(s) |
jgreenha@ucsd.edu
|
| Organization name |
The Salk Institute
|
| Department |
MNL-H
|
| Street address |
10010 N. Torrey Pines Rd.
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE6238 |
Mechanisms of Aging in Senescence-Accelerated Mice |
|
