|
| Status |
Public on Aug 08, 2014 |
| Title |
H3K27Ac_MF.ucsc |
| Sample type |
SRA |
| |
|
| Source name |
Bone Marrow Macrophages
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Bone Marrow Macrophages
passages: In vivo FACS purified cells
strain: C57BL/6
chip antibody: H3K27Ac
|
| Growth protocol |
Cells were fixated and FACS sorted according to stablished markers
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody.
A new protocol (iChIP) was designed to perform iCHIP from small cell numbers
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 1500 |
| |
|
| Data processing |
Basecalls performed using CASAVA version 1.4
Genome_build: mm9
Supplementary_files_format_and_content: wig files were generated using …; Scores represent …
|
| |
|
| Submission date |
Jul 21, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Ido Amit |
| E-mail(s) |
ido.amit@weizmann.ac.il
|
| Phone |
972-8-9343338
|
| Organization name |
Weizmann Institute of Science
|
| Department |
Immunology
|
| Street address |
234 Herzl st.
|
| City |
Rehovot |
| ZIP/Postal code |
760001 |
| Country |
Israel |
| |
|
| Platform ID |
GPL18480 |
| Series (2) |
| GSE59636 |
Chromatin state dynamics during blood formation (ChIP-Seq) |
| GSE60103 |
Chromatin state dynamics during blood formation |
|
| Relations |
| BioSample |
SAMN02929028 |
| SRA |
SRX658384 |
