|
| Status |
Public on Nov 14, 2006 |
| Title |
Tobacco_abuse_3 |
| Sample type |
RNA |
| |
|
| Source name |
lymphoblast cell line
|
| Organism |
Homo sapiens |
| Characteristics |
Tobacco abuse
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from the cell lines using our standard procedures using an Invitrogen RNA purification kit (Invitrogen, Carlsbad, CA) according to the instructions provided by the manufacturer and stored in Ambion RNA storage solution at –80o
|
| Label |
DIG-UTP
|
| Label protocol |
Digoxigenin-UTP labeled cRNA was generated and amplified from 2 μg of total RNA from each sample using Applied Biosystems Chemiluminescent RT-IVT Labeling Kit v 1.0 (P/N 4340472) according to the manufacturer’s protocol (P/N 4339629).
|
| |
|
| Hybridization protocol |
Array hybridization was performed for 16 hrs at 55 oC. Chemiluminescence detection, image acquisition and analysis were performed using Applied Biosystems Chemiluminescence Detection Kit (P/N 4342142) and Applied Biosystems 1700 Chemiluminescent Microarray Analyzer (P/N 4338036) following the manufacturer’s protocol (P/N 4339629).
|
| Scan protocol |
image acquisition and analysis were performed using Applied Biosystems Chemiluminescence Detection Kit (P/N 4342142) and Applied Biosystems 1700 Chemiluminescent Microarray Analyzer (P/N 4338036) following the manufacturer’s protocol (P/N 4339629).
|
| Description |
Tobacco abuse in the Iowa Adoption Studies
|
| Data processing |
Images were auto-gridded and the chemiluminescent signals were quantified, corrected for background, and finally, spot- and spatially-normalized using the Applied Biosystems 1700 Chemiluminescent Microarray Analyzer software v 1.1 (P/N 4336391).
|
| |
|
| Submission date |
Nov 09, 2006 |
| Last update date |
Nov 13, 2006 |
| Contact name |
Gi-yung Ryu |
| E-mail(s) |
giyung.ryu@gmail.com
|
| Organization name |
University of Iowa
|
| Department |
Neurosurgery
|
| Street address |
EMRB 200A
|
| City |
Iowa City |
| State/province |
IA |
| ZIP/Postal code |
52242 |
| Country |
USA |
| |
|
| Platform ID |
GPL1426 |
| Series (1) |
| GSE6264 |
Transcriptional Profiling of Subjects from the Iowa Adoption Studies |
|
