|
Status |
Public on Nov 18, 2006 |
Title |
Breast Cancer Pre-Treatment 1 |
Sample type |
RNA |
|
|
Source name |
Tumor mammary tissue 1 previous to treatment.
|
Organism |
Homo sapiens |
Characteristics |
Core-needle biopsy was taken from primary breast tumors at diagnosis day prior to the first course of doxorubicin plus cyclophosphamide.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA was prepared according to the standard CodeLink protocol from 2 ug total RNA
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 37C on Human Whole Genome CodeLink Bioarray. Stain and post-hybridization washes were performed according to the manufacturer's instructions.
|
Scan protocol |
Bioarrays were scanned with a GenePix Personal 4100A scanner.
|
Description |
Gene expression data from a primary breast tumor previous to receive hydralazine and magnesium valproate prior to the first course of doxorubicin plus cyclophosphamide.
|
Data processing |
Expression values were globally normalized to the median expression value of the whole array spots. To circumvent division by zero, values below three times background value were taken as 5.
|
|
|
Submission date |
Nov 16, 2006 |
Last update date |
Nov 17, 2006 |
Contact name |
Carlos Pérez-Plasencia |
E-mail(s) |
car_plas@yahoo.com
|
Phone |
+(52) 55 56280400
|
Fax |
+(52) 55 56280486
|
URL |
http://www.incan.edu.mx/
|
Organization name |
INSTITUTO NACIONAL DE CANCEROLOGIA
|
Department |
BASIC RESEARCH
|
Lab |
EPIGENETICS
|
Street address |
Av. San Fernando 22
|
City |
México |
State/province |
D.F. |
ZIP/Postal code |
14080 |
Country |
Mexico |
|
|
Platform ID |
GPL2895 |
Series (1) |
GSE6304 |
Gene Expression Profiles of Epigenetic treatment in primary breast cancer. |
|