|
| Status |
Public on Nov 05, 2014 |
| Title |
Control-2 |
| Sample type |
RNA |
| |
|
| Source name |
Liver, scrambled control ASO
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
gender: Male
age: 25 weeks
chow: HFD
|
| Treatment protocol |
Mice received intraperitoneal injections of antisense oligonucleotide (ASO) directed against Mogat1 or a scrambled control ASO (25 mg/kg body weight; ISIS Pharmaceuticals, Carlsbad, CA) twice a week for three weeks. Treatments were initiated after 14 weeks of high fat diet feeding.
|
| Growth protocol |
C57BL/6J male mice were fed chow providing 60% of calories from fatty acids (D12492, Research Diets Inc) starting at 6 weeks of age.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total liver mRNA was extracted with RNA Bee (Isotex Diagnostics, Friendswood, TX) based on the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
RNA transcripts were amplified by T7 linear amplification (MessageAmp TotalPrep amplification kit; ABI-Ambion).
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
sample name: 937
replicate 2
|
| Data processing |
The data were normalised using quantile normalisation (background was also subtracted).
|
| |
|
| Submission date |
Aug 12, 2014 |
| Last update date |
Sep 15, 2023 |
| Contact name |
Jun Yoshino |
| E-mail(s) |
jyoshino@med.shimane-u.ac.jp
|
| Organization name |
Faculty of Medicine, Shimane University
|
| Street address |
89-1 Enya-cho
|
| City |
Izumo |
| State/province |
Shimane |
| ZIP/Postal code |
693-8501 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE60349 |
Inhibiting Monoacylglycerol Acyltransferase 1 Ameliorates Hepatic Metabolic Abnormalities, but not Inflammation and Injury in Mice |
|
