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Status
Public on Dec 01, 2014
Title
P2
Sample type
RNA
Source name
ISG15 deficient patient
Organism
Homo sapiens Characteristics
cell type: H tert Fibroblasts
Treatment protocol
HTert-immortalized fibroblasts from C1, C6, C16, P1, P2 and P3 were treated with 1000U of IFN-α2b for 12 h, washed with PBS and left to rest for 36 h, after which relative mRNA levels were assessed
Extracted molecule
total RNA
Extraction protocol
RNAeasy kits from Qiagen
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA.
Hybridization protocol
Standard Affymetrix protocol was perfomed
Scan protocol
GeneChips were scanned using standard GeneArray Scanner.
Description
P2
Data processing
Affymetrix Console and TAC
Submission date
Aug 12, 2014
Last update date
Dec 01, 2014
Contact name
Dusan Bogunovic
E-mail(s)
dusan.bogunovic@mssm.edu Organization name
Icahn School of Medicine at Mount Siani
Department
Microbiology
Street address
1468 Madison Avenue,
City
New York
State/province
New York
ZIP/Postal code
10029
Country
USA
Platform ID
GPL17586 Series (1)
GSE60359 ISG15 in regulation of interferon responses
Data table header descriptions ID_REF VALUE log2 rma-gene-full
Data table ID_REF VALUE TC01000001.hg.1
6.82568
TC01000002.hg.1
5.67964
TC01000003.hg.1
3.08232
TC01000004.hg.1
2.81054
TC01000005.hg.1
8.0292
TC01000006.hg.1
11.8754
TC01000007.hg.1
11.7943
TC01000008.hg.1
6.16038
TC01000009.hg.1
2.75437
TC01000010.hg.1
4.29057
TC01000011.hg.1
6.62903
TC01000012.hg.1
4.1777
TC01000013.hg.1
9.70668
TC01000014.hg.1
3.12334
TC01000015.hg.1
5.41954
TC01000016.hg.1
4.23174
TC01000017.hg.1
6.85415
TC01000018.hg.1
8.16763
TC01000019.hg.1
6.52077
TC01000020.hg.1
7.16473
70523 1655 Kbytes .Supplementary file Size Download File type/resource GSM1474243_16_HTA_2.0_Dusan_08132013_HTA-2_0_.CEL.gz
24.8 Mb
(ftp) (http) CEL
GSM1474243_16_HTA_2.0_Dusan_08132013_HTA-2_0_.RMA.rma-gene-full.chp.gz
457.4 Kb
(ftp) (http) CHP
Processed data included within Sample table Processed data provided as supplementary file
