|
| Status |
Public on Nov 06, 2015 |
| Title |
normal2 [mRNA] |
| Sample type |
RNA |
| |
|
| Source name |
Fresh frozen tumor
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: adjacent normal tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from cell pellets using mirVana kit (Ambion/Life Technologies) according to the manufacturer’s instructions. Total RNA was eluted in 100 µl H2O, concentration measured on NanoDrop ND-1000.
|
| Label |
Cy3
|
| Label protocol |
mRNA was labeled with Cy-3 according to the manual One-Color Microarray-Based Gene Expression Analysis, Low Input Quick Amp Labeling (version 6.5, May 2010) from Agilent Technologies, using 100 ng total RNA as input.
|
| |
|
| Hybridization protocol |
For the expression arrays Cy-3-labeled cRNA was hybridized according to the manual One-Color Microarray-Based Gene Expression Analysis, Low Input Quick Amp Labeling (version 6.5, May 2010) from Agilent Technologies.
|
| Scan protocol |
The slides were scanned immediately after washing on the Agilent Microarray Scanner G2565C using protocol GE1_107_Sep09 for the expression microarrays and protocol.
|
| Description |
Gene expression of adjacent normal tissue 2
|
| Data processing |
The scanned images were analyzed using Feature Extraction Software 10.7.3.1 (Agilent Technologies) using default parameters. The expression profiles were backgroundcorrected and quantile normalized for mRNA. Filtering of controls, unannotated genes and low expressed probes was done.
|
| |
|
| Submission date |
Sep 02, 2014 |
| Last update date |
Nov 06, 2015 |
| Contact name |
Vandana Sandhu |
| E-mail(s) |
vansan@rr-research.no
|
| Organization name |
Radiumhospitalet, Oslo
|
| Department |
Department of Genetics
|
| Street address |
Ullernchausseen 70
|
| City |
Oslo |
| State/province |
oslo |
| ZIP/Postal code |
0310 |
| Country |
Norway |
| |
|
| Platform ID |
GPL14550 |
| Series (2) |
| GSE60979 |
mRNA and miRNA expression profiles for fresh frozen periampullary adenocarcinomas (pancreatobiliary and intestinal type) and adjacent normal tissues. [mRNA] |
| GSE60980 |
mRNA and miRNA expression profiles for fresh frozen periampullary adenocarcinomas (pancreatobiliary and intestinal type) and adjacent normal tissues. |
|
