|
| Status |
Public on Dec 07, 2014 |
| Title |
TF-pMSCVpuro-GNB1K89E-no Cytokine withdrawl-replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
TF-pMSCVpuro-GNB1K89E-no Cytokine withdrawl
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: TF-1
transduced with: pMSCVpuro-GNB1K89E
time point: 12 after maintained in GM-CSF
cytokine withdrawl: no
|
| Growth protocol |
Total RNA was extracted in biological triplicates using Trizol from stably transfected TF-1 cells (empty vector control vs. K89E) with or without 12 hour cytokine starvation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were homogenized in Trizol and RNA was extracted according to standard protocols.
|
| Label |
biotin
|
| Label protocol |
cRNA was biotinylated according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized overnight according to the Affymetrix protocol.
|
| Scan protocol |
GCS3000 (Affymetrix)
|
| Description |
46_TFk89eplus11
|
| Data processing |
The raw cel files were converted into probeset-specific expression values using Affymetrix RMA summarization method using Brainarray cdf annotations (hugene10st_Hs_ENSG.cdf, version 16) with the ExpressionFileCreator module (version 11.14) embedded in the GenePattern environment (Reich et al., 2006).
|
| |
|
| Submission date |
Sep 02, 2014 |
| Last update date |
Dec 07, 2014 |
| Contact name |
Andrew Lane |
| E-mail(s) |
aalane@partners.org
|
| Phone |
617-632-4589
|
| Organization name |
Dana-Farber Cancer Institute
|
| Street address |
450 Brookline Ave
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL19145 |
| Series (1) |
| GSE60990 |
Mutations in G protein beta subunits promote transformation and kinase inhibitor resistance. |
|
