|
| Status |
Public on Sep 09, 2014 |
| Title |
Glioma P45 induction 5 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Glioma P45 induction (acute dissection, no cell purification)
|
| Organism |
Mus musculus |
| Characteristics |
cell type: OPC
treatment: Glioma induction
mutation induction age: P45
|
| Treatment protocol |
None
|
| Growth protocol |
None
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mice were decapitated and brains dissected. Tumor was identified by fluorescence under dissecting scope and acutely dissected. Tissue was placed in Trizol and total RNA was purified.
|
| Label |
Cy5
|
| Label protocol |
Test samples were labeled with Cy5 fluorophore. Reference samples were labeled with Cy3 fluorophore.
|
| |
|
| Channel 2 |
| Source name |
common reference (RNA pool of four WT P17 mouse brain neocortex)
|
| Organism |
Mus musculus |
| Characteristics |
reference: WT P17 mouse brain neocortex
|
| Treatment protocol |
None
|
| Growth protocol |
None
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mice were decapitated and brains dissected. Tumor was identified by fluorescence under dissecting scope and acutely dissected. Tissue was placed in Trizol and total RNA was purified.
|
| Label |
Cy3
|
| Label protocol |
Test samples were labeled with Cy5 fluorophore. Reference samples were labeled with Cy3 fluorophore.
|
| |
|
| |
| Hybridization protocol |
Agilent microarray protocol
|
| Scan protocol |
After scanning the images by using a GenePix 4000B scanner, the intensity values were extracted by using the Agilent Feature Extraction Software.
|
| Description |
12948 (5)
|
| Data processing |
Probe sequences were downloaded from the Agilent website and aligned to a transcript sequence database consisting of 49,040 Mus musculus genes from Ensembl57. After removing probes that did not align, or aligned with more than 5 mismatches (n = 19,194), or probes that aligned to multiple genes (n = 2,283), 23,006 probes representing 17,585 genes remained. Expression profiles were quantile normalized and log transformed
|
| |
|
| Submission date |
Sep 09, 2014 |
| Last update date |
Sep 09, 2014 |
| Contact name |
Rui Pedro Galvao |
| E-mail(s) |
galvao@uoregon.edu
|
| Organization name |
University of Oregon
|
| Street address |
1229 University of Oregon
|
| City |
Eugene |
| State/province |
Oregon |
| ZIP/Postal code |
97403 |
| Country |
USA |
| |
|
| Platform ID |
GPL19163 |
| Series (1) |
| GSE61282 |
Transformation of quiescent adult oligodendrocyte precursor cells into malignant glioma through a multi-step reactivation process |
|
