|
| Status |
Public on Oct 07, 2014 |
| Title |
MMTV-Cre:Rbp53 primary tumor TMCRP-1 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
pooled mouse tail DNA
|
| Organism |
Mus musculus |
| Characteristics |
strain background: mixed
tissue: tail
source: reference DNA (non-tumor bearing)
|
| Treatment protocol |
Genomic DNA obtained from primary tumors were used for aCGH analysis to determine copy number alterations following combined loss of Rb/p53
|
| Growth protocol |
Samples from independent primary mammary tumors from MMTV-Cre:Rbp53,MMTV-Cre:p53 and Ad-Cre:Rbp53 models were frozen and kept in -80 degree.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNAs were purified using DNAeasy kits (Qiagen)
|
| Label |
Cy3
|
| Label protocol |
The Invitrogen BioPrime CGH labeling kit was used (Cat#18094011) according to manufacturer's protocol
|
| |
|
| Channel 2 |
| Source name |
Primary mammary tumor Rbp53 deleted
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Primary mammary tumor
strain background: mixed
tumor model: MMTV-Cre (Transplant)
|
| Treatment protocol |
Genomic DNA obtained from primary tumors were used for aCGH analysis to determine copy number alterations following combined loss of Rb/p53
|
| Growth protocol |
Samples from independent primary mammary tumors from MMTV-Cre:Rbp53,MMTV-Cre:p53 and Ad-Cre:Rbp53 models were frozen and kept in -80 degree.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNAs were purified using DNAeasy kits (Qiagen)
|
| Label |
Cy5
|
| Label protocol |
The Invitrogen BioPrime CGH labeling kit was used (Cat#18094011) according to manufacturer's protocol
|
| |
|
| |
| Hybridization protocol |
2 ug of each DNA sample was hybridized according to the 40-hour manual protocol described by the manufacturer
|
| Scan protocol |
The arrays were scanned at 5 micron resolution in an Agilent scanner
|
| Description |
Tumor DNA is being compared to pooled tail DNA from animals of same mixed background
|
| Data processing |
The images were processed with Agilent feature extraction version 9.5.1.1
|
| |
|
| Submission date |
Oct 06, 2014 |
| Last update date |
Oct 07, 2014 |
| Contact name |
Robert Jones |
| E-mail(s) |
rjones12@uoguelph.ca
|
| Organization name |
University of Guelph
|
| Department |
Biomedical Sciences
|
| Street address |
50 Stone Rd
|
| City |
Guelph |
| ZIP/Postal code |
N1G 2W1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL10448 |
| Series (1) |
| GSE62101 |
aCGH data of Rb/p53 deficient mammary tumors |
|
