|
| Status |
Public on Oct 18, 2015 |
| Title |
E2s_PROG_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
T47D breast cancer cells, 10nM estradiol and progesterone treated 16h, rep 2
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: T47D
cell type: breast cancer cell line
|
| Treatment protocol |
hormone treatment: Plated in hormone stripped medium (Phenol red free RPMI1640 supplemented with 10% dextran charcoal stripped FCS) for 56h, followed by treatment with 10nM estradiol and 10nM progesterone for 16h. Replicate 2
Cells were plated at 500,000 cells per well in 6 well plates in phenol red free RPMI 1640 supplemented with 10% dextran charcoal stripped FBS and incubated for 56h. Cells were subsequently treated for 16h with the nominated hormone(s).
|
| Growth protocol |
Cells were grown in RPMI 1640 supplemented with 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using a Qiagen Rneasy RNA extraction kit, incorporating Dnase on-column digestion according to manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
biotinylated cDNA was prepared using 1ug of total RNA according to standard Illumina protocols
|
| |
|
| Hybridization protocol |
Standard Illumina hybridisation protocol
|
| Scan protocol |
Standard Illumina Scanning protocol
|
| Data processing |
Data was normalised using cubic spline normalisation in bead studio. For publication, data was normalised using variance stabilisation normalisation, corrected with ComBat, filtered to likely expressed transcripts and then subjected to linear model fitting as implemented in the bioconductor Limma package in R.
|
| |
|
| Submission date |
Oct 10, 2014 |
| Last update date |
Oct 18, 2015 |
| Contact name |
Eleanor F Need |
| E-mail(s) |
eleanor.need@adelaide.edu.au
|
| Organization name |
The University of Adelaide
|
| Department |
Discipline of Surgery
|
| Lab |
Cancer Biology Group
|
| Street address |
28 Woodville Rd
|
| City |
Woodville South |
| State/province |
SA |
| ZIP/Postal code |
5011 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6171 |
| Series (1) |
| GSE62243 |
Cotreatment effects of estrogen with progestogens or dihydrotestosterone in the breast cancer cell line T-74D |
|
| Relations |
| BioSample |
SAMN02851190 |
