|
| Status |
Public on Jan 06, 2007 |
| Title |
E-edc3D, biological replicate 4 |
| Sample type |
RNA |
| |
|
| Source name |
edc3 deletion strain CFY25
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Genotype, edc3::URA3
|
| Treatment protocol |
none
|
| Growth protocol |
Cells were grown in YEPD at 30°C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Hot phenol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Yeast YG-S98 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
edc3 deletion strain CFY25
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250.
|
| |
|
| Submission date |
Jan 04, 2007 |
| Last update date |
Jan 09, 2007 |
| Contact name |
Feng He |
| E-mail(s) |
feng.he@umassmed.edu
|
| Phone |
508-856-3931
|
| Organization name |
University of Massachusetts Medical School
|
| Department |
Microbiology and Physiological Systems
|
| Street address |
368 Plantation Street
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01655 |
| Country |
USA |
| |
|
| Platform ID |
GPL90 |
| Series (1) |
| GSE6647 |
Expression analysis of edc3 cell |
|
