|
| Status |
Public on Nov 01, 2015 |
| Title |
Tregs stimulated in the presence of IL-27. Replicate #1 |
| Sample type |
RNA |
| |
|
| Source name |
regulatory T cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: regulatory T cells
|
| Treatment protocol |
rIL-27 (10ng/ml)
|
| Growth protocol |
CD4+Foxp3GFP+ 5x10^5 cells/ml stimulated with CD3/28 coated dynabeads added IL-2 (100u/ml) for 48hrs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with QIAGEN RNeasy mini kit followed by clean-up and DNase I treatment in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
48hrs +IL-27_1
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Nov 19, 2014 |
| Last update date |
Nov 01, 2015 |
| Contact name |
Yibayiri Osee Sanogo |
| Organization name |
Cleveland Clinic Foundation
|
| Department |
Genomics Core
|
| Street address |
9500 S Euclid Ave
|
| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44195 |
| Country |
USA |
| |
|
| Platform ID |
GPL19441 |
| Series (1) |
| GSE63455 |
IL-27 stimulation in Foxp3+ Tregs improves suppressive function and therapeutic efficacy |
|
