|
| Status |
Public on Nov 27, 2014 |
| Title |
MUNC deprived differentiating cells |
| Sample type |
RNA |
| |
|
| Source name |
C2C12 cell line_siMUNC_differentiating
|
| Organism |
Mus musculus |
| Characteristics |
cell line: C2C12
knockdown: siMUNC
|
| Treatment protocol |
Differentiation: when cells were 80% confluent medium was changed to 2% horse serum containing DMEM medium. Cells were collected 3 days after differentiation.
|
| Growth protocol |
C2C12 cells were cultured in 10% FBS high glucose DMEM medium (proliferating cells)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using TRIZOL method
|
| Label |
biotin
|
| Label protocol |
GeneChip® WT Terminal Labeling and Hybridization User Manual for use with the Ambion® WT Expression Kit
|
| |
|
| Hybridization protocol |
The Ambion® WT Expression Kit For Affymetrix® GeneChip® Whole Transcript (WT) Expression Arrays
|
| Scan protocol |
Affymetrix scanner 3000 with 7G upgrade, Fluidics Stations 450; standard scanner settings
|
| Description |
differentiating cells
C12C12 DM3 siMUNC
|
| Data processing |
Affymetrix Expression Console Console software and Microsoft Excel
|
| |
|
| Submission date |
Nov 26, 2014 |
| Last update date |
Nov 27, 2014 |
| Contact name |
Anindya Dutta |
| E-mail(s) |
ad8q@virginia.edu
|
| Organization name |
University Of Virginia
|
| Department |
Biochemistry & Molecular Genetics
|
| Lab |
Dutta Lab
|
| Street address |
1300 Jefferson Park Ave
|
| City |
Charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22908 |
| Country |
USA |
| |
|
| Platform ID |
GPL6096 |
| Series (1) |
| GSE63673 |
MUNC downregulation decreases muscle differentiation |
|
