|
| Status |
Public on Oct 12, 2015 |
| Title |
DUCaP cells treated for 24 hours with EtOH, experiment 3 |
| Sample type |
RNA |
| |
|
| Source name |
DUCaP
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: EtOH
time: 24 h
cell line: DUCaP
date of experiment: 25.05.2010
|
| Treatment protocol |
Cells were cultured in the absence (EtOH) or presence of 1nM R1881 for 8 and 24 hours, respectively.
|
| Growth protocol |
Cells (LNCaP, VCaP, DuCaP) were grown on polylysine-coated (Sigma) petridishes in RPMI1640 supplemented with 10% charcoal-stripped FCS overnight.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using TRI-reagent (Sigma) according to the manufacturer`s protocol. After DNA digestion with DnaseI (Qiagen), RNA was purified using Rneasy kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Target RNA was labeled by addition of biotin-linked deoxynucleotides with terminal deoxynucleotidyl transferase according to the manufacturer's (Affymetrix) protocols. Washing and staining was performed in an Affymetrix 450S fluidics station.
|
| |
|
| Hybridization protocol |
Target samples were hybridized to Human Gene 1.0 ST v1 microarrays according to the manufacturer's protocols in a Affymetrix hybridization oven.
|
| Scan protocol |
Microarrays were scanned in an Affymetrix 3000 scanner.
|
| Description |
DUCaP cells treated for 24 hours with EtOH, experiment 3
|
| Data processing |
Raw data was preprocessed using the RMA method and a custom CDF from the Brainarray group (ENSG CDF, version 13).
|
| |
|
| Submission date |
Nov 28, 2014 |
| Last update date |
Oct 13, 2015 |
| Contact name |
Johannes Rainer |
| E-mail(s) |
johannes.rainer@eurac.edu
|
| Organization name |
Eurac Researc
|
| Department |
Institute for Biomedicine
|
| Lab |
Biomedical Informatics
|
| Street address |
Via A. Volta 21
|
| City |
Bolzano |
| ZIP/Postal code |
39100 |
| Country |
Italy |
| |
|
| Platform ID |
GPL16786 |
| Series (1) |
| GSE63693 |
Prostate Cancer Risk SNPs enriched in Androgen Receptor Binding Sites |
|
