|
| Status |
Public on Nov 29, 2014 |
| Title |
XP+dox day2 1/21 |
| Sample type |
RNA |
| |
|
| Source name |
HCOXP, doxycycline treated, 2 days
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Lung
gender: male
cell line: H1299
|
| Treatment protocol |
The cells were treated with doxycycline (1 ug/ml) for either 2 or 7 days after drug administration.
|
| Growth protocol |
The cells were maintained in Dulbecco modified Eagles' Medium (DMEM) with 10% fetal bovine serum supplied, and were incubated in humidified 37 degree Celsius incubator with 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The cells were lysed by TRIzol reagent and froze. After thawing, the lysates were extracted by Zymo spin column. Total RNAs were then subjected to quantified and validated by Nanodrop ND-1000.
|
| Label |
cy3
|
| Label protocol |
not provided
|
| |
|
| Hybridization protocol |
not provided
|
| Scan protocol |
Fluorescent images were obtained using Agilent Technologies Scanner G2505C US84303579
|
| Description |
miRNA expression of HCOXP cells with doxycycline treatment for 2 days
|
| Data processing |
Agilent Feature Extraction Software (v 10.1.1.1) was used for background subtraction and LOWESS normalization. Data analysis was performed using GenomicWorkbench v5.0(Agilent Technology)
|
| |
|
| Submission date |
Nov 28, 2014 |
| Last update date |
Nov 29, 2014 |
| Contact name |
Liang-Ting Lin |
| E-mail(s) |
aceliang@gmail.com
|
| Organization name |
National Yang-Ming University
|
| Street address |
No.155, Sec.2, Li-Nong St., Beitou District
|
| City |
Taipei |
| State/province |
--- |
| ZIP/Postal code |
11164 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL14767 |
| Series (1) |
| GSE63699 |
The expression pattern of microRNAs during the overexpression of cofilin1 |
|
