|
Status |
Public on Jan 01, 2017 |
Title |
Hns – 120 min |
Sample type |
SRA |
|
|
Source name |
Escherichia coli CSH50 Δhns
|
Organism |
Escherichia coli |
Characteristics |
strain: CSH50 genotype: delta-hns time: 120 minutes
|
Growth protocol |
LB medium, 37°C, airated, constant pH 7.5
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA extraction Kit GATC
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
|
|
Description |
temporal gene expression hns mutant 120 min sample grown in LB in a fermenter @ 37°C, constant pH 7.5 and air saturated partial oxygen pressure
|
Data processing |
GATC software for read mapping on MG1655 verified with in-house pipeline Gene wise natural spline interpolation of temporal data from initial time points to a dataset of 10 minutes steps (see processed data files). Expression values at the time points of the raw data are not affected by the interpolation. gene expression sum normalized to 1 at each time point Supplementary_files_format_and_content: expression of genes in time, rows = genes, cols = time points
|
|
|
Submission date |
Jan 23, 2015 |
Last update date |
May 15, 2019 |
Contact name |
Patrick Sobetzko |
E-mail(s) |
patrick.sobetzko@synmikro.uni-marburg.de
|
Organization name |
Philipps-Universität Marburg LOEWE-Zentrum für Synthetische Mikrobiologie
|
Department |
Department of Chromosome Biology
|
Lab |
AG Waldminghaus
|
Street address |
Hans-Meerwein-Straße
|
City |
Marburg |
ZIP/Postal code |
35043 |
Country |
Germany |
|
|
Platform ID |
GPL14548 |
Series (1) |
GSE65244 |
Temporal gene expression in Escherichia coli |
|
Relations |
BioSample |
SAMN03292481 |
SRA |
SRX851925 |