|
| Status |
Public on Feb 07, 2007 |
| Title |
DMSO 12 hr 2 |
| Sample type |
RNA |
| |
|
| Source name |
DMSO 12 hr
|
| Organism |
Homo sapiens |
| Characteristics |
DMSO 12 hr
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Normal human epidermal keratinocytes (NHEKs) were treated with DMSO or 9 (alias PP-2) (0.5 uM) and harvested at 12, 24, 36 and 48 h. Total RNA from each sample was isolated and purified with the RNeasy Mini Kit (Qiagen). Total RNA was examined on an Agilent Bioanalyzer and the 28S/18S ratio exceeded 2.0 for all samples.
|
| Label |
biotin
|
| Label protocol |
cDNA synthesis and amplification employed the Affymetrix 3' IVT protocol.
|
| |
|
| Hybridization protocol |
hybridization followed standard Affymetrix protocols while array washing used a GNF custom machine
|
| Scan protocol |
standard Affymetrix procedures using the GeneChip 3000 scanner
|
| Description |
DMSO 12 hr
|
| Data processing |
gcRMA
|
| |
|
| Submission date |
Feb 01, 2007 |
| Last update date |
Feb 07, 2007 |
| Contact name |
John R Walker |
| E-mail(s) |
jwalker@gnf.org
|
| Phone |
858-812-1636
|
| Organization name |
Genomics Institute of the Novartis Research Foundation
|
| Lab |
Genetics Core
|
| Street address |
10675 John Jay Hopkins
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92121 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE6932 |
Identification and characterization of small molecule inducers of epidermal keratinocyte differentiation |
|
