|
| Status |
Public on Feb 13, 2015 |
| Title |
ILC1 PLZF+/+ rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse Liver
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
genotype: PLZF+/+
cell type: ILC1
age: 4-10 weeks
tissue: Liver
gating: CD3e- NK1.1+ CD49a+ DX5-
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy mini kit (Qiagen) to extract total RNA from cells stored in Trizol (Life Technologies)
|
| Label |
Cy3
|
| Label protocol |
Standard Illumina labeling protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
ILC1 WT replicate 1
|
| Data processing |
The non-normalized array data were extracted using GenomeStudio (version GSGX 1.9.0). Background correction, variance stabilization, and quantile normalization were performed in R/Bioconductor using the 'lumi' package.
|
| |
|
| Submission date |
Feb 12, 2015 |
| Last update date |
Feb 13, 2015 |
| Contact name |
Albert Bendelac |
| Organization name |
The University of Chicago
|
| Department |
Pathology
|
| Street address |
929 East 57th Street, GCIS W506
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platform ID |
GPL18752 |
| Series (1) |
| GSE65898 |
PLZF expression maps the early stages of ILC1 lineage development |
|
